IB Metabolic Core

 

IB Metabolic Core

A nexus for connecting researchers using integrative methods to study metabolic physiology of animals ranging from humans and mice to bugs. From whole organism respirometry and metabolic flux analyses to cell culture and mitochondrial physiology, we facilitate campus collaborations in metabolic physiology of animals and make equipment and expertise more freely available to the UC Berkeley community. By participating in the IB Metabolic Core, you agree to uphold our community values.

Community values:

  • We share our time, expertise and resources freely and collaboratively to enhance the UC Berkeley metabolic physiology community. If someone in the community asks for help, we provide it to the best of our ability.
  • If someone has helped us, we consider what is the appropriate recognition for their contributions to our project; whether that be an acknowledgement, co-authorship, or a simple thank you. We are generous in our consideration and appreciation of our colleagues.
  • We take care of our equipment and resources. We learn protocols carefully before using equipment, and ask if we’re not sure. If anything happens to a piece of equipment, we immediately tell the contact person. We keep our eyes open for upcoming issues or maintenance required on equipment and work proactively to maintain clean, tidy and functional spaces.
  • Safety is of the utmost importance at all times. We find out all relevant safety protocols before beginning any new experiments. If we see anything unsafe, we immediately report it to the contact person for the lab we are working in.
  • We create an inclusive and collaborative environment. We treat each other with the utmost respect at all times. If we witness any incidents that make anyone feel unwelcome, we immediately report them to the contact person for the lab we are working in.
  • We learn the individual culture of the lab we are working in by asking the members about expectations and customs, and aim to abide by those norms even if they are different to our own lab.
  • All equipment is available first to the members of the hosting lab, usage is subject to availability and not to be assumed without communication with the contact person. Consistent violation of community values may lead to loss of use of community equipment.
  • Consumable and reagent costs will be born by the user unless otherwise determined, with arrangements made through discussion with the hosting lab.

Participating laboratories:

Exercise Physiology Laboratory (P.I. George Brooks): http://ib.berkeley.edu/labs/brooks/

Vazquez-Medina lab (P.I. José Pablo Vázquez-Medina): https://www.vazquezmedinalab.com/

Williams Ecological and Evolutionary Physiology laboratory (P.I. Caroline Williams): http://www.cmwilliamslab.com/

Current major equipment available for shared use:

Equipment Function Location Contact
LiCor7000 dual channel CO2 analyzer

 

Measure respiratory gas exchange Williams lab

5117 VLSB

cmw@berkeley.edu
Sable Systems Oxzilla II dual channel O2 analyzer

 

Measure respiratory gas exchange Williams lab

5117 VLSB

cmw@berkeley.edu
Foxbox (field portable CA-10 CO2 analyzer plus Oxzilla I single channel O2 analyzer with integrated pump system)

 

Measure respiratory gas exchange Williams lab

5117 VLSB

cmw@berkeley.edu
Iatroscan TLC-FID Lipid composition, polar and neutral lipids Williams lab

5117 VLSB

 

cmw@berkeley.edu
BioTek Synergy HTX plate multimode plate readers

 

UV-Vis, Fluorescence, or Luminescence spectroscopy in plate format Williams lab

5117 VLSB

cmw@berkeley.edu
Oroboros Instruments O2k microrespirometry systems (2 units, 2 chambers each)

 

Mitochondrial bioenergetics of isolated mitochondria or permeabilized tissue Williams lab

5117 VLSB

cmw@berkeley.edu
Zeiss Axiobserver 7 Inverted Microscope System with 3 fluorescence channels (blue, green, red) and 3 objectives (10X, 20x, 63x) suitable for examination of subcellular structures including mitochondria

 

Vazquez-Medina lab

5047 VLSB

jpv-m@berkeley.edu
Baker InVivo2 Physiological Cell Culture Workstation

 

Short- and Long-term cell culture at controlled oxygen environments Vazquez-Medina lab

5047 VLSB

jpv-m@berkeley.edu
Etaluma LS620 Inverted Microscope Compact system with 3 fluorescence channels and 2 Objectives (20x and 40x). Can be used inside of a hypoxia station or other incubator

 

Vazquez-Medina lab

5047 VLSB

jpv-m@berkeley.edu
Molecular Devices SpectraMax M3 plate reader Monochromator-based UV-Vis, Fluorescence, or Luminescence spectroscopy with temperature control in cuvette and plate format

 

Vazquez-Medina lab

5045 VLSB

jpv-m@berkeley.edu
Agilent Seahorse XFp Analyzer (6 sample wells) Measure oxidative phosphorylation or glycotylic energy production in cells, tissues, or isolated mitochondria. Brooks lab

5101 VLSB


gbrooks@berkeley.edu